Browsing by Author "Lundin, Mikael"
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- ItemFive years of experience teaching pathology to dental students using the WebMicroscope.(2011-04-14) Szymas, Janusz; Lundin, MikaelWe describe development and evaluation of the user friendly web based virtual microscopy WebMicroscope for teaching and learning dental students basic and oral pathology Traditional students microscopes were replaced by computer workstations
- ItemOn-chip imaging of Schistosoma haematobium eggs in urine for diagnosis by computer vision.(2013-12-16) Linder, Ewert; Grote, Anne; Varjo, Sami; Linder, Nina; Lebbad, Marianne; Lundin, Mikael; Diwan, Vinod; Hannuksela, Jari; Lundin, JohanBACKGROUND Microscopy being relatively easy to perform at low cost is the universal diagnostic method for detection of most globally important parasitic infections As quality control is hard to maintain misdiagnosis is common which affects both estimates of parasite burdens and patient care Novel techniques for high resolution imaging and image transfer over data networks may offer solutions to these problems through provision of education quality assurance and diagnostics Imaging can be done directly on image sensor chips a technique possible to exploit commercially for the development of inexpensive mini microscopes Images can be transferred for analysis both visually and by computer vision both at point of care and at remote locations METHODS PRINCIPAL FINDINGS Here we describe imaging of helminth eggs using mini microscopes constructed from webcams and mobile phone cameras The results show that an inexpensive webcam stripped off its optics to allow direct application of the test sample on the exposed surface of the sensor yields images of Schistosoma haematobium eggs which can be identified visually Using a highly specific image pattern recognition algorithm 4 out of 5 eggs observed visually could be identified CONCLUSIONS SIGNIFICANCE As proof of concept we show that an inexpensive imaging device such as a webcam may be easily modified into a microscope for the detection of helminth eggs based on on chip imaging Furthermore algorithms for helminth egg detection by machine vision can be generated for automated diagnostics The results can be exploited for constructing simple imaging devices for low cost diagnostics of urogenital schistosomiasis and other neglected tropical infectious diseases
- ItemWeb-based virtual microscopy in teaching and standardizing Gleason grading.(2005-05-13) Helin, Henrik; Lundin, Mikael; Lundin, Johan; Martikainen, Paula; Tammela, Teuvo; Helin, Heikki; van der Kwast, Theo; Isola, JormaGleason grading forms the basis of prognostic and therapeutic assessment in prostatic carcinoma despite its subjective nature and substantial interobserver variation The accuracy of Gleason grading can be improved by the use of educational tools such as reference images However conventional microscopy images are of limited educational value because it is neither possible to view the sample at different magnifications nor to navigate into different areas of the specimen This limitation can be overcome by the use of virtual microscopy which allows viewing entire digitized microscope slides We created an interactive Web site www webmicroscope net gleason featuring a comprehensive set of prostatic needle biopsies as virtual slides which can be viewed with a standard Web browser Internet Explorer or Netscape To evaluate the validity of Web based virtual microscopy for Gleason grading an experienced uropathologist TK scored a series of 62 biopsies from the original glass slides and 6 weeks later from virtual slides on the Web site using an ordinary desktop computer The intraobserver agreement was excellent with identical Gleason scores found in 48 of the 62 cases kappa 0 73 The 14 remaining scores differed only by 1 point on the Gleason scale 2 10 The virtual slides were viewed by 2 other uropathologists PM and HH with interobserver kappa coefficients ranging from 0 55 to 0 62 which is within the range of previously reported studies using glass slides The 3 uropathologists Gleason scores were included as reference scores on the Web site which now serves as a publicly open platform for self testing and learning of Gleason grading We conclude that Web based virtual microscopy is a promising new tool for teaching and standardizing Gleason grading